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International Starch Institute
Science Park Aarhus, Denmark
ISI 06 Table 4a  - Determination of Dry Matter in 42% Fructose Syrup by Refractive Index

1. Scope The table is applicable to 42% fructose syrup with 0.05% ash on dry matter. LT 24 Nov. 1997
2. Principle The refractive index of sample is transformed to dry matter by a table.
3. Apparatus 3.1 Refractometer with thermostat
4. Procedure Adjust temperature of refractometer (3.1) to 20, 30, 45 or 60 oC. +/- 0.2 oC
Adjust sample temperature to that chosen for the refractometer.
Place a few drops of sample on fixed prism and close instrument Work fast
Read the refractive index (nD) to nearest 0.0002 unit. Clean prisms immediately after.
5. Calculation Find dry matter (DM) in percent of sample (average results of two samples with no decimal) by using table: Correct eventually for differing DE

DM%

20 oC

30 oC

45 oC

60 oC

0

1,33299

1,33101

1,32985

1,32725

2

1,33588

1,33401

1,33265

1,33002

4

1,33877

1,33701

1,33547

1,33279

6

1,34171

1,34001

1,33833

1,33561

8

1,34470

1,34301

1,34123

1,33848

10

1,34772

1,34601

1,34418

1,34140

12

1,35080

1,34901

1,34718

1,34436

14

1,35392

1,35201

1,35022

1,34737

16

1,35709

1,35501

1,35331

1,35042

18

1,36030

1,35801

1,35645

1,35353

20

1,36356

1,36201

1,35963

1,35668

22

1,36687

1,36501

1,36287

1,35988

24

1,37023

1,36801

1,36615

1,36314

26

1,37363

1,37201

1,36949

1,36644

28

1,37709

1,37501

1,37287

1,36980

30

1,38059

1,37901

1,37631

1,37320

32

1,38415

1,38201

1,37979

1,37666

34

1,38776

1,38601

1,38333

1,38017

36

1,39141

1,38901

1,38692

1,38373

38

1,39512

1,39301

1,39057

1,38735

40

1,39889

1,39701

1,39426

1,39102

42

1,40270

1,40001

1,39802

1,39475

44

1,40657

1,40401

1,40182

1,39853

46

1,41049

1,40801

1,40568

1,40237

48

1,41447

1,41201

1,40960

1,40627

50

1,41851

1,41601

1,41358

1,41022

52

1,42260

1,42001

1,41761

1,41423

54

1,42675

1,42401

1,42170

1,41830

56

1,43095

1,42901

1,42585

1,42243

58

1,43522

1,43301

1,43006

1,42662

60

1,43954

1,43701

1,43433

1,43087

62

1,44392

1,44101

1,43866

1,43519

64

1,44837

1,44601

1,44305

1,43956

66

1,45287

1,45001

1,44750

1,44400

68

1,45744

1,45501

1,45202

1,44851

70

1,46207

1,45901

1,45660

1,45308

72

1,46677

1,46401

1,46125

1,45771

74

1,47152

1,46901

1,46596

1,46241

76

1,47635

1,47401

1,47074

1,46718

78

1,48124

1,47901

1,47559

1,47202

80

1,48620

1,48301

1,48051

1,47693

82

1,49122

1,48801

1,48549

1,48191

84

1,49632

1,49401

1,49055

1,48696
6. Notes
7. Reference International Standard: ISO 1743

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Keywords: laboratory method determination dry matter substance starch glucose maltose fructose syrup refraction refractive index