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International Starch Institute
Science Park Aarhus, Denmark
ISI 06 Table 4b  - Determination of Dry Matter in 55% Fructose Syrup by Refractive Index

1. Scope The table is applicable to 55% fructose syrup with 0.05% ash on dry matter. LT 24 Nov. 1997
2. Principle The refractive index of sample is transformed to dry matter by a table.
3. Apparatus 3.1 Refractometer with thermostat
4. Procedure Adjust temperature of refractometer (3.1) to 20, 30, 45 or 60 oC. +/- 0.2 oC
Adjust sample temperature to that chosen for the refractometer.
Place a few drops of sample on fixed prism and close instrument Work fast
Read the refractive index (nD) to nearest 0.0002 unit. Clean prisms immediately after.
5. Calculation Find dry matter (DM) in percent of sample (average results of two samples with no decimal) by using table: Correct eventually for differing DE

DM%

20 oC

30 oC

45 oC

60 oC

0

1,33299

1,33194

1,32985

1,32725

2

1,33587

1,33479

1,33265

1,33002

4

1,33876

1,33764

1,33546

1,33279

6

1,34170

1,34054

1,33832

1,33561

8

1,34468

1,34349

1,34122

1,33848

10

1,34771

1,34648

1,34417

1,34139

12

1,35078

1,34952

1,34717

1,34435

14

1,35389

1,35260

1,35020

1,34736

16

1,35706

1,35573

1,35329

1,35041

18

1,36027

1,35891

1,35643

1,35351

20

1,36352

1,36213

1,35961

1,35666

22

1,36683

1,36540

1,36284

1,35986

24

1,37018

1,36872

1,36612

1,36310

26

1,37358

1,37209

1,36944

1,36640

28

1,37703

1,37551

1,37282

1,36975

30

1,38054

1,37897

1,37625

1,37314

32

1,38409

1,38249

1,37973

1,37659

34

1,38769

1,38606

1,38326

1,38009

36

1,39134

1,38968

1,38684

1,38364

38

1,39505

1,39336

1,39048

1,38725

40

1,39881

1,39708

1,39416

1,39091

42

1,40262

1,40086

1,39791

1,39462

44

1,40649

1,40470

1,40170

1,39839

46

1,41041

1,40859

1,40555

1,40222

48

1,41438

1,41253

1,40946

1,40610

50

1,41841

1,41653

1,41343

1,41004

52

1,42250

1,42059

1,41745

1,41403

54

1,42665

1,42471

1,42153

1,41808

56

1,43085

1,42888

1,42566

1,42220

58

1,43511

1,43311

1,42986

1,42637

60

1,43944

1,43740

1,43412

1,43060

62

1,44382

1,44175

1,43844

1,43490

64

1,44826

1,44617

1,44281

1,43925

66

1,45277

1,45064

1,44726

1,44367

68

1,45733

1,45518

1,45176

1,44816

70

1,46197

1,45978

1,45633

1,45271

72

1,46666

1,46445

1,46097

1,45732

74

1,47142

1,46918

1,46567

1,46200

76

1,47625

1,47398

1,47043

1,46675

78

1,48115

1,47885

1,47527

1,47156

80

1,48611

1,48378

1,48017

1,47645

82

1,49114

1,48878

1,48514

1,48141

84

1,49624

1,49386

1,49019

1,48643
6. Notes
7. Reference International Standard: ISO 1743

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Keywords: laboratory method determination dry matter substance starch glucose maltose fructose syrup refraction refractive index