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International Starch Institute
Science Park Aarhus, Denmark
ISI 06 Table 4c  - Determination of Dry Matter in 90% Fructose Syrup by Refractive Index

1. Scope The table is applicable to 90% fructose syrup with 0.05% ash on dry matter. LT 24 Nov. 1997
2. Principle The refractive index of sample is transformed to dry matter by a table.
3. Apparatus 3.1 Refractometer with thermostat
4. Procedure Adjust temperature of refractometer (3.1) to 20, 30, 45 or 60 oC. +/- 0.2 oC
Adjust sample temperature to that chosen for the refractometer.
Place a few drops of sample on fixed prism and close instrument Work fast
Read the refractive index (nD) to nearest 0.0002 unit. Clean prisms immediately after.
5. Calculation Find dry matter (DM) in percent of sample (average results of two samples with no decimal) by using table: Correct eventually for differing DE

DM%

20 oC

30 oC

45 oC

60 oC

0

1,33299

1,33194

1,32985

1,32725

2

1,33586

1,33477

1,33263

1,32998

4

1,33875

1,33762

1,33542

1,33274

6

1,34169

1,34051

1,33827

1,33553

8

1,34467

1,34345

1,34115

1,33838

10

1,34769

1,34644

1,34409

1,34127

12

1,35076

1,34947

1,34707

1,34420

14

1,35388

1,35255

1,35009

1,34719

16

1,35704

1,35567

1,35316

1,35022

18

1,36025

1,35884

1,35628

1,35329

20

1,36351

1,36206

1,35945

1,35642

22

1,36681

1,36532

1,36267

1,35960

24

1,37017

1,36864

1,36593

1,36282

26

1,37357

1,37200

1,36925

1,36609

28

1,37702

1,37542

1,37261

1,36942

30

1,38052

1,37888

1,37603

1,37279

32

1,38408

1,38239

1,37949

1,37622

34

1,38768

1,38596

1,38301

1,37970

36

1,39133

1,38957

1,38657

1,38323

38

1,39504

1,39324

1,39019

1,38681

40

1,39880

1,39696

1,39387

1,39044

42

1,40261

1,40074

1,39759

1,39413

44

1,40648

1,40456

1,40138

1,39788

46

1,41040

1,40844

1,40521

1,40168

48

1,41437

1,41238

1,40910

1,40553

50

1,41840

1,41637

1,41305

1,40944

52

1,42249

1,42042

1,41705

1,41341

54

1,42663

1,42453

1,42111

1,41743

56

1,43083

1,42869

1,42523

1,42152

58

1,43508

1,43291

1,42940

1,42566

60

1,43940

1,43719

1,43364

1,42986

62

1,44378

1,44153

1,43793

1,43412

64

1,44821

1,44593

1,44229

1,43845

66

1,45271

1,45039

1,44670

1,44283

68

1,45727

1,45491

1,45118

1,44728

70

1,46189

1,45949

1,45572

1,45179

72

1,46657

1,46414

1,46033

1,45636

74

1,47132

1,46885

1,46500

1,46100

76

1,47613

1,47362

1,46973

1,46570

78

1,48100

1,47846

1,47453

1,47047

80

1,48595

1,48337

1,47940

1,47531

82

1,49096

1,48835

1,48433

1,48022

84

1,49603

1,49339

1,48933

1,48519
6. Notes
7. Reference International Standard: ISO 1743

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Keywords: laboratory method determination dry matter substance starch glucose maltose fructose syrup refraction refractive index