Starch Links:
Determination of Starch in Tubers and Roots by Under Water Weight [ISI
Method No. 13]
Determination of Starch in Potatoes by Under Water Weight according
to EU-direction [EU-Method]
AMYLOSE
AND AMYLOPECTIN Normal native starches consist of a mixture of 15-30
per cent. amylose and 70-85 per cent. amylopectin. Amylose approximately
between 40 000 and 340 000, the chains containing 250 to 2000 anhydroglucose
units. Amylopectin is considered to be composed of anhydroglucose chains
with many branch points; the molecular weight may reach as high as 80
000 000 (Re. WHO). Amylose is an unbranched chain which is coiled in
the shape of a helix. If iodine is added to a solution containing amylose
molecules, the iodine inserts itself into the helix making it rigid.
This changes the color of the starch mixture to blue or purple depending
on the length of the amylose molecule. Amylopectin is a branching molecule
which does not form a helical coil. Thus the iodine is not able to bind
to the starch molecule. Amylose contributes to the gelling property
of starch whereas amylopectin contributes high viscosity. This classic
statement, however, may not be entirely valid. Both properties are used
in the preparation of foods.
In amylose the C6H10O5 groups are arranged
in a continuous but curled chain somewhat like a coil of rope; in the
second kind, amylopectin, considerable side-branching of the molecule
occurs.
Starch is manufactured by green plants during the process of photosynthesis. It forms part of the cell walls in plants, constitutes part of rigid plant fibres, and serves as a kind of energy storage for plants, because its oxidation to carbon dioxide and water releases energy. The granules of starch present in any plant have size, shape, and markings characteristic of the species of plant in which the starch is made.
Starch is almost insoluble in cold water and in alcohol, but with boiling water it gives a colloidal suspension that may form a jelly on cooling. Hot water changes starch slowly into smaller molecules. This reaction, an example of hydrolysis, is catalyzed by acids and by some enzymes giving still simpler molecules, the ultimate products being maltose, C12H22O11, a disaccharide, and glucose, C6H12O6, a monosaccharide.
The digestion of starch in the human body takes the following course: the hydrolysis begins in the mouth under the action of salivary ptyalin, but is completed in the small intestine. The body does not immediately use all the glucose absorbed from the digestion of starch, but converts much of it to glycogen, which is stored in the liver. (Glycogen, called animal starch, has a structure nearly identical with that of amylopectin.) As the body requires glucose, hydrolysis of glycogen releases it into the bloodstream. Glycogen provides an energy reserve for animals in the same way that ordinary starch does for plants.
Amylopectin (Fig. 2):
Amylose Content and Granule Size of Various Starches
| Starch Source | % Amylose | Granule Size Range (m) | Average size m |
| Waxy Rice | 0 | 2 - 15 | 6 |
| High Amylose Corn | 70 | 4 - 20 | 10 |
| Corn | 28 | 5 - 25 | 14 |
| Cassava | 17 | 3 - 30 | 14 |
| Waxy Sorghum | 0 | - | - |
| Wheat | 26 | 3 - 35 | 7 and 20 |
| Sweet Potato | 18 | 4 - 40 | 19 |
| Arrowroot | 21 | 9 - 40 | 23 |
| Sago | 26 | 15 - 50 | 33 |
| Potato | 20 | 10 - 100 | 36 |